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验证数据展示

  • IHC staining of human gliomas using 11190-2-AP

    IHC staining of human gliomas using 11190-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP (PMF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 11190-2-AP

    WB analysis of Jurkat using 11190-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

PMF1 Polyclonal antibody

PMF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11190-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PMF-1, Polyamine-modulated factor 1, polyamine modulated factor 1, PMF 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11190-2-AP targets PMF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PMF1 fusion protein Ag1672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polyamine-modulated factor 1
别名 PMF-1, Polyamine-modulated factor 1, polyamine modulated factor 1, PMF 1
计算分子量 19 kDa
观测分子量 23 kDa
GenBank蛋白编号BC033656
基因名称 PMF1
Gene ID (NCBI) 11243
RRIDAB_2064906
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P1K2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PMF1 antibody 11190-2-APDownload protocol
IHC protocol for PMF1 antibody 11190-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols