验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, multi-tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10666-1-AP targets PMM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PMM2 fusion protein Ag1031 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphomannomutase 2 |
| 别名 | phosphomannomutase 2, EC:5.4.2.8 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC008310 |
| 基因名称 | PMM2 |
| Gene ID (NCBI) | 5373 |
| RRID | AB_2166973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15305 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IHC protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IF protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IP protocol for PMM2 antibody 10666-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thromb Haemost N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity
| ||
J Proteome Res Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese. | ||
Mol Genet Metab Complex metabolic disharmony in PMM2-CDG paves the way to new therapeutic approaches | ||
J Inherit Metab Dis Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling. | ||
Dis Model Mech Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG. | ||
PLoS One Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2. |
