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验证数据展示

  • WB analysis using 83456-6-RR

    WB analysis using 83456-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83456-6-RR (PMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83456-6-RR

    IHC staining of human ovary cancer using 83456-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83456-6-RR (PMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83456-6-RR

    IF Staining of HeLa using 83456-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HeLa using 83456-6-RR

    FC experiment of HeLa using 83456-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PMS1 Recombinant antibody (83456-6-RR, Clone:240408D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83456-6-RR

    Affinity and Kinetic Characterization of 83456-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83456-6-RR against Human PMS1 were performed. The affinity constant is 0.367 nM.

PMS1 Recombinant antibody

PMS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83456-6-RR

产品说明书

CloneNo. 240408D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PMSL1, MLH2, hPMS1, HNPCC3, 240408D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, K-562 cells, Jurkat cells, MOLT-4 cells, HL-60 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, Caco-2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83456-6-RR targets PMS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PMS1 fusion protein Ag28132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
别名 PMSL1, MLH2, hPMS1, HNPCC3, 240408D9
观测分子量 106 kDa
GenBank蛋白编号BC096331
基因名称 PMS1
Gene ID (NCBI) 5378
RRIDAB_3671092
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP54277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

实验方案

Product Specific Protocols
WB protocol for PMS1 antibody 83456-6-RRDownload protocol
IHC protocol for PMS1 antibody 83456-6-RRDownload protocol
IF protocol for PMS1 antibody 83456-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols