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验证数据展示

  • WB analysis using 18164-1-AP

    WB analysis using 18164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 18164-1-AP

    IHC staining of human colon cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 18164-1-AP

    IHC staining of human ovary cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PMS2 Polyclonal antibody

PMS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18164-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18164-1-AP targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PMS2 fusion protein Ag12651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
别名 PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-
计算分子量 862 aa, 96 kDa
观测分子量 117 kDa, 100 kDa
GenBank蛋白编号BC093921
基因名称 PMS2
Gene ID (NCBI) 5395
RRIDAB_2167145
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834)

实验方案

Product Specific Protocols
WB protocol for PMS2 antibody 18164-1-APDownload protocol
IHC protocol for PMS2 antibody 18164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols