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PMS2 Polyclonal antibody

PMS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18164-1-AP

Print datasheet

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

验证数据展示

  • WB analysis using 18164-1-AP

    WB analysis using 18164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 18164-1-AP

    IHC staining of human colon cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 18164-1-AP

    IHC staining of human ovary cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, PC-3 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18164-1-AP targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PMS2 fusion protein Ag12651 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Synonyms PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-
Calculated Molecular Weight 862 aa, 96 kDa
Observed Molecular Weight 117 kDa, 100 kDa
GenBank Accession NumberBC093921
Gene Symbol PMS2
Gene ID (NCBI) 5395
RRIDAB_2167145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834)

实验方案

Product Specific Protocols
WB protocol for PMS2 antibody 18164-1-APDownload protocol
IHC protocol for PMS2 antibody 18164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols