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  • 概述
  • 经过测试的应用
  • 产品信息
  • 发表文章 (2)
  • 实验方案

PMVK Polyclonal antibody

PMVK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15674-1-AP

Print datasheet

Synonyms

hPMK, HUMPMKI, phosphomevalonate kinase, PMK, PMKA, PMKASE, PMKI, PMVK

验证数据展示

  • WB analysis using 15674-1-AP

    WB analysis using 15674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15674-1-AP (PMVK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15674-1-AP

    IHC staining of human liver cancer using 15674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15674-1-AP (PMVK Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 15674-1-AP

    IHC staining of human liver cancer using 15674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15674-1-AP (PMVK Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15674-1-AP targets PMVK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PMVK fusion protein Ag8141 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphomevalonate kinase
Synonyms hPMK, HUMPMKI, phosphomevalonate kinase, PMK, PMKA, PMKASE, PMKI, PMVK
Calculated Molecular Weight 192 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007694
Gene Symbol PMVK
Gene ID (NCBI) 10654
RRIDAB_2299910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PMVK antibody 15674-1-APDownload protocol
IHC protocol for PMVK antibody 15674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB

Sci Rep

Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.

Authors - Jiuxiang Wang
View Article
humanWB

Sci Rep

MicroRNA-874 targets phosphomevalonate kinase and inhibits cancer cell growth via the mevalonate pathway

Authors - Alimasi Aersilan
View Article