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PNCK Polyclonal antibody

PNCK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13712-1-AP

Print datasheet

Synonyms

BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK

验证数据展示

  • WB analysis using 13712-1-AP

    WB analysis using 13712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13712-1-AP targets PNCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PNCK fusion protein Ag4650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pregnancy up-regulated non-ubiquitously expressed CaM kinase
Synonyms BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC033746
Gene Symbol PNCK
Gene ID (NCBI) 139728
RRIDAB_10642952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P2M8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNCK antibody 13712-1-APDownload protocol
IHC protocol for PNCK antibody 13712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols