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验证数据展示

  • WB analysis using 13712-1-AP

    WB analysis using 13712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 40x lens).

PNCK Polyclonal antibody

PNCK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13712-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:9000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13712-1-AP targets PNCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PNCK fusion protein Ag4650 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pregnancy up-regulated non-ubiquitously expressed CaM kinase
别名 BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK
计算分子量 47 kDa
观测分子量 38 kDa, 45 kDa
GenBank蛋白编号BC033746
基因名称 PNCK
Gene ID (NCBI) 139728
RRIDAB_10642952
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P2M8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNCK antibody 13712-1-APDownload protocol
IHC protocol for PNCK antibody 13712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols