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验证数据展示

  • IHC staining of human breast cancer using 13558-1-AP

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13558-1-AP

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 13558-1-AP

    WB analysis of Jurkat using 13558-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PNKP Polyclonal antibody

PNKP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DNA 5 kinase/3 phosphatase, PNK, PNKP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13558-1-AP targets PNKP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PNKP fusion protein Ag4421 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polynucleotide kinase 3'-phosphatase
别名 DNA 5 kinase/3 phosphatase, PNK, PNKP
计算分子量 521 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC033822
基因名称 PNKP
Gene ID (NCBI) 11284
RRIDAB_2877958
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T60
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNKP antibody 13558-1-APDownload protocol
IHC protocol for PNKP antibody 13558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols