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PNLDC1 Polyclonal antibody

PNLDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25559-1-AP

Print datasheet

Synonyms

PNLDC1

验证数据展示

  • IHC staining of human testis using 25559-1-AP

    IHC staining of human testis using 25559-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25559-1-AP (PNLDC1 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 25559-1-AP

    WB analysis using 25559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25559-1-AP (PNLDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25559-1-AP targets PNLDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, silkworm
Immunogen PNLDC1 fusion protein Ag22074 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Synonyms PNLDC1
Calculated Molecular Weight 520 aa, 60 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC112246
Gene Symbol PNLDC1
Gene ID (NCBI) 154197
RRIDAB_2880131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NA58
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNLDC1, also named as Poly(A)-specific ribonuclease PARN-like domain-containing protein 1, is a 520 amino acid protein, which belongs to the CAF1 family. PNLDC1 as a membrane protein localizes in the membrane.

实验方案

Product Specific Protocols
WB protocol for PNLDC1 antibody 25559-1-APDownload protocol
IHC protocol for PNLDC1 antibody 25559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
silkwormWB

Cell

Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms.

Authors - Natsuko Izumi
View Article
humanIHC

J Ovarian Res

A risk model of gene signatures for predicting platinum response and survival in ovarian cancer.

Authors - Siyu Chen
View Article