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验证数据展示

  • WB analysis of PC-3 using 68415-1-Ig

    WB analysis of PC-3 using 68415-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 68415-1-Ig

    IF Staining of PC-3 using 68415-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PNMA1 antibody (68415-1-Ig, Clone: 2D8C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PNMA1 Monoclonal antibody

PNMA1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68415-1-Ig

产品说明书

CloneNo. 2D8C6

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, T-47D cells, LNCaP cells, rat testis tissue
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68415-1-Ig targets PNMA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 PNMA1 fusion protein Ag33143 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 paraneoplastic antigen MA1
别名 Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6
计算分子量 353 aa, 40 kDa
观测分子量37 kDa
GenBank蛋白编号BC039577
基因名称 PNMA1
Gene ID (NCBI) 9240
RRIDAB_3085131
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8ND90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNMA1 antibody 68415-1-IgDownload protocol
IF protocol for PNMA1 antibody 68415-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols