验证数据展示
经过测试的应用
| Positive WB detected in | HT-1376 cells, TT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21770-1-AP targets PNMA5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PNMA5 fusion protein Ag16414 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | paraneoplastic antigen like 5 |
| 别名 | paraneoplastic antigen like 5, KIAA1934 |
| 计算分子量 | 448 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC101112 |
| 基因名称 | PNMA5 |
| Gene ID (NCBI) | 114824 |
| RRID | AB_3669374 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PV4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNMA5 is a member of the paraneoplastic Ma antigen (PNMA) family proteins which share relatively conserved protein sequences and are involved in the regulation of growth, apoptosis, or fate determination in different cell types (PMID: 29378289). PNMA5 appears to be involved in the association areas of the neocortex during primate evolution (PMID: 19366867). PNMA5 has been reported to play roles in the pathological development and metastasis of some cancers, including colorectal cancer, ovarian cancer, and lung cancer (PMID: 35559417; 35117731; 34136487).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNMA5 antibody 21770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |