• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of K-562 using 13217-1-AP

    WB analysis of K-562 using 13217-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13217-1-AP (PNMT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-12 using 13217-1-AP

    IP experiment of PC-12 using 13217-1-AP

    IP result of anti-PNMT (IP:13217-1-AP, 4ug; Detection:13217-1-AP 1:300) with PC-12 cells lysate 2400ug.

PNMT Polyclonal antibody

PNMT Polyclonal Antibody for WB, IP, ELISA
Cat No. 13217-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13217-1-AP targets PNMT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PNMT fusion protein Ag4098 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phenylethanolamine N-methyltransferase
别名 PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28
计算分子量 282 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC037246
基因名称 PNMT
Gene ID (NCBI) 5409
RRIDAB_2167781
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phenylethanolamine N-methyltransferase(PNMT), the final enzyme in catecholamine biosynthesis, converts norepinephrine to epinephrine, thereby serving as a marker of the adrenergic phenotype.

实验方案

Product Specific Protocols
WB protocol for PNMT antibody 13217-1-APDownload protocol
IP protocol for PNMT antibody 13217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.

Authors - Shin Takasawa
View Article
humanWB

Front Endocrinol (Lausanne)

Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension

Authors - He Ma
View Article