验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18266-1-AP targets Pinin in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Pinin fusion protein Ag6643 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pinin, desmosome associated protein |
| 别名 | SR-like protein, PNN, DRS, Domain-rich serine protein, Desmosome-associated protein |
| 计算分子量 | 82 kDa |
| 观测分子量 | 82 kDa, 140 kDa |
| GenBank蛋白编号 | BC062602 |
| 基因名称 | Pinin |
| Gene ID (NCBI) | 5411 |
| RRID | AB_10642138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H307 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Pinin antibody 18266-1-AP | Download protocol |
| IF protocol for Pinin antibody 18266-1-AP | Download protocol |
| IP protocol for Pinin antibody 18266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Mol Oncol LncRNA AATBC regulates Pinin to promote metastasis in nasopharyngeal carcinoma.
| ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
Exp Physiol Methyltransferase like 3 enhances pinin mRNA stability through N6 -methyladenosine modification to augment tumourigenesis of colon adenocarcinoma | ||
Oncotarget Pinin associates with prognosis of hepatocellular carcinoma through promoting cell proliferation and suppressing glucose deprivation-induced apoptosis. | ||
Cell Rep Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion |