验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, mouse spleen tissue, K-562 cells, A549 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human liver cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
21059-1-AP targets PNO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PNO1 fusion protein Ag14752 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | partner of NOB1 homolog (S. cerevisiae) |
| 别名 | KHRBP1, PNO1, RNA binding protein PNO1 |
| 计算分子量 | 252 aa, 28 kDa |
| 观测分子量 | 28-35 kDa |
| GenBank蛋白编号 | BC008304 |
| 基因名称 | PNO1 |
| Gene ID (NCBI) | 56902 |
| RRID | AB_10734582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRX1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The function of PNO1 remains largely unknown in mammal. PNO1 plays a role in proteasome and ribosome neogenesis in yeast.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNO1 antibody 21059-1-AP | Download protocol |
| IHC protocol for PNO1 antibody 21059-1-AP | Download protocol |
| IP protocol for PNO1 antibody 21059-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway
| ||
Heliyon Pan-cancer exploration of PNO1: A prospective prognostic biomarker with ties to immune infiltration |
