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PNPLA3 Monoclonal antibody, PBS Only

PNPLA3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2B11

Cat no : 67369-1-PBS

Print datasheet

Synonyms

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

验证数据展示

  • IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

    IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPLA3 antibody (67369-1-Ig, Clone: 1D2B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

    WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67369-1-Ig (PNPLA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

产品信息

67369-1-PBS targets PNPLA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, pig
Immunogen PNPLA3 fusion protein Ag27407 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name patatin-like phospholipase domain containing 3
Synonyms Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC065195
Gene Symbol PNPLA3
Gene ID (NCBI) 80339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NST1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)