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验证数据展示

  • IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

    IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPLA3 antibody (67369-1-Ig, Clone: 1D2B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

    WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67369-1-Ig (PNPLA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

PNPLA3 Monoclonal antibody, PBS Only

PNPLA3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67369-1-PBS

产品说明书

CloneNo. 1D2B11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67369-1-PBS targets PNPLA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Rat, pig
免疫原 PNPLA3 fusion protein Ag27407 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 patatin-like phospholipase domain containing 3
别名 Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3
观测分子量 53 kDa
GenBank蛋白编号BC065195
基因名称 PNPLA3
Gene ID (NCBI) 80339
RRIDAB_2882619
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NST1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)