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验证数据展示

  • WB analysis of HEK-293 using 14261-1-AP

    WB analysis of HEK-293 using 14261-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment using 14261-1-AP

    IP experiment using 14261-1-AP

    IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells.

  • IF Staining of HepG2 using 14261-1-AP

    IF Staining of HepG2 using 14261-1-AP

    Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PNPLA6 Polyclonal antibody

PNPLA6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14261-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

sws, SPG39, Patatin-like phospholipase domain-containing protein 6, NTEMND, NTE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PNPLA6 Ready-To-Use IHC Kit. PNPLA6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14261-1-AP targets PNPLA6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 PNPLA6 fusion protein Ag5546 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 patatin-like phospholipase domain containing 6
别名 sws, SPG39, Patatin-like phospholipase domain-containing protein 6, NTEMND, NTE
计算分子量 150 kDa
观测分子量 150 kDa
GenBank蛋白编号BC051768
基因名称 PNPLA6
Gene ID (NCBI) 10908
RRIDAB_2283965
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PNPLA6 antibody 14261-1-APDownload protocol
IF protocol for PNPLA6 antibody 14261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

FMRP targets distinct mRNA sequence elements to regulate protein expression.

Authors - Ascano Manuel M Jr
View Article

Mol Biol Cell

Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum.

Authors - Alexandre Luscher
View Article