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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

    WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

    IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

PNPT1 Monoclonal antibody, PBS Only

PNPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68309-1-PBS

产品说明书

CloneNo. 3F2A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PNPT1 Ready-To-Use IHC Kit. PNPT1 primary antibody included.

产品信息

68309-1-PBS targets PNPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PNPT1 fusion protein Ag6290 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 polyribonucleotide nucleotidyltransferase 1
别名 3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1
计算分子量 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC053660
基因名称 PNPT1
Gene ID (NCBI) 87178
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCS8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.