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PODXL2 Polyclonal antibody

PODXL2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16383-1-AP

Print datasheet

Synonyms

Endoglycan, PODLX2, podocalyxin like 2, Podocalyxin like protein 2, PODXL2

验证数据展示

  • WB analysis of mouse pancreas using 16383-1-AP

    WB analysis of mouse pancreas using 16383-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 16383-1-AP

    IP experiment of K-562 using 16383-1-AP

    IP result of anti-PODXL2 (IP:16383-1-AP, 4ug; Detection:16383-1-AP 1:300) with K-562 cells lysate 4000ug.

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16383-1-AP targets PODXL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PODXL2 fusion protein Ag9374 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name podocalyxin-like 2
Synonyms Endoglycan, PODLX2, podocalyxin like 2, Podocalyxin like protein 2, PODXL2
Calculated Molecular Weight605aa,65 kDa; 529aa,57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC019330
Gene Symbol PODXL2
Gene ID (NCBI) 50512
RRIDAB_2166112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ53
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PODXL2 antibody 16383-1-APDownload protocol
IP protocol for PODXL2 antibody 16383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Med

Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance.

Authors - Ingrid G Winkler
View Article