验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells |
| Positive IHC detected in | mouse liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
17764-1-AP targets POFUT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | POFUT2 fusion protein Ag12148 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein O-fucosyltransferase 2 |
| 别名 | C21orf80, FUT13, KIAA0958, O FucT 2, Peptide O fucosyltransferase 2, POFUT2, protein O fucosyltransferase 2 |
| 计算分子量 | 429 aa, 50 kDa |
| 观测分子量 | 49-50 kDa |
| GenBank蛋白编号 | BC064623 |
| 基因名称 | POFUT2 |
| Gene ID (NCBI) | 23275 |
| RRID | AB_1959662 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2G5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POFUT2 antibody 17764-1-AP | Download protocol |
| IHC protocol for POFUT2 antibody 17764-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder | ||
Cell Death Dis LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface. | ||
Schizophr Res Altered fucosyltransferase expression in the superior temporal gyrus of elderly patients with schizophrenia. | ||
Dev Biol Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. | ||
Methods Mol Biol Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays. | ||
J Cancer Res Clin Oncol Emerging glyco-risk prediction model to forecast response to immune checkpoint inhibitors in colorectal cancer
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