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POLD1 Polyclonal antibody
POLD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15646-1-AP targets POLD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine |
| Immunogen | POLD1 fusion protein Ag8178 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | polymerase (DNA directed), delta 1, catalytic subunit 125kDa |
| Synonyms | CDC2, POLD, POLD1 |
| Calculated Molecular Weight | 1107 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC008800 |
| Gene Symbol | POLD1 |
| Gene ID (NCBI) | 5424 |
| RRID | AB_2252572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28340 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLD1 antibody 15646-1-AP | Download protocol |
| IHC protocol for POLD1 antibody 15646-1-AP | Download protocol |
| IP protocol for POLD1 antibody 15646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
Science MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. | ||
Nat Microbiol Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation. | ||
Nat Commun Hepatitis B virus cccDNA is formed through distinct repair processes of each strand. | ||
Hum Mol Genet Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. | ||
Hum Mol Genet ISCA1 mutation in a patient with infantile-onset leukodystrophy causes defects in mitochondrial [4Fe-4S] proteins. |
