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验证数据展示

  • WB analysis using 29765-1-AP

    WB analysis using 29765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29765-1-AP

    IHC staining of human colon using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLD2 antibody included
  • IF Staining of HeLa using 29765-1-AP

    IF Staining of HeLa using 29765-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

POLD2 Polyclonal antibody

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29765-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNA polymerase delta subunit 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, SGC-7901 cells, U-251 cells, mouse colon tissue, mouse pancreas tissue, rat colon tissue
Positive IHC detected inhuman colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLD2 Ready-To-Use IHC Kit. POLD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29765-1-AP targets POLD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen POLD2 fusion protein Ag31521 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed), delta 2, regulatory subunit 50kDa
Synonyms DNA polymerase delta subunit 2
Calculated Molecular Weight 51 kDa
Observed Molecular Weight48-51 kDa
GenBank Accession NumberBC000459
Gene Symbol POLD2
Gene ID (NCBI) 5425
RRIDAB_2918344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49005
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

实验方案

Product Specific Protocols
WB protocol for POLD2 antibody 29765-1-APDownload protocol
IHC protocol for POLD2 antibody 29765-1-APDownload protocol
IF protocol for POLD2 antibody 29765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols