验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, SGC-7901 cells, U-251 cells, mouse colon tissue, mouse pancreas tissue, rat colon tissue |
Positive IHC detected in | human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29765-1-AP targets POLD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | POLD2 fusion protein Ag31521 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | polymerase (DNA directed), delta 2, regulatory subunit 50kDa |
别名 | DNA polymerase delta subunit 2 |
计算分子量 | 51 kDa |
观测分子量 | 48-51 kDa |
GenBank蛋白编号 | BC000459 |
基因名称 | POLD2 |
Gene ID (NCBI) | 5425 |
RRID | AB_2918344 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P49005 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.
实验方案
Product Specific Protocols | |
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WB protocol for POLD2 antibody 29765-1-AP | Download protocol |
IHC protocol for POLD2 antibody 29765-1-AP | Download protocol |
IF protocol for POLD2 antibody 29765-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |