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POLD3 Polyclonal antibody

POLD3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 21935-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy POLD3 Ready-To-Use IHC Kit. POLD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21935-1-AP targets POLD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 POLD3 fusion protein Ag16570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA-directed), delta 3, accessory subunit
别名 DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3
计算分子量 466 aa, 51 kDa
观测分子量 51-70 kDa
GenBank蛋白编号BC108908
基因名称 POLD3
Gene ID (NCBI) 10714
RRIDAB_2878949
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15054
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLD3 antibody 21935-1-APDownload protocol
IHC protocol for POLD3 antibody 21935-1-APDownload protocol
IP protocol for POLD3 antibody 21935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

Break-induced replication promotes fragile telomere formation.

Authors - Zhe Yang
  • KO Validated
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
  • KD Validated
humanWB

J Adv Res

Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA

Authors - Xi Dong
human

Mol Cell

Mechanisms of insertions at a DNA double-strand break

Authors - Jaewon Min
mouseWB

Nucleic Acids Res

Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model

Authors - Nada Kojak
mouseWB

Nucleic Acids Res

Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres

Authors - Jennifer J Lee
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