POLG Polyclonal antibody

POLG Polyclonal Antibody for WB, ELISA
Cat No. 14110-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

EC:4.2.99.-, EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14110-1-AP targets POLG in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen POLG fusion protein Ag5256 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed), gamma
Synonyms EC:4.2.99.-, EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase
Calculated Molecular Weight 140 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC042571
Gene Symbol POLG
Gene ID (NCBI) 5428
RRIDAB_3669178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLG is the catalytic subunit of DNA polymerase gamma solely responsible for the replication of mitochondrial DNA (mtDNA). POLG replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers, and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).

实验方案

Product Specific Protocols
WB protocol for POLG antibody 14110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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