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POLK Polyclonal antibody

POLK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14455-1-AP

Print datasheet

Synonyms

DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ

验证数据展示

  • WB analysis using 14455-1-AP

    WB analysis using 14455-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14455-1-AP (POLK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14455-1-AP targets POLK in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen POLK fusion protein Ag6034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed) kappa
Synonyms DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC050718
Gene Symbol POLK
Gene ID (NCBI) 51426
RRIDAB_2165839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLK, also named as DINB1, belongs to the DNA polymerase type-Y family. POLK is a DNA polymerase specifically involved in DNA repair. POLK Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, POLK inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK is ubiquitously expressed in tissues, but is most abundant in testis, adrenal gland and ovary (PMID: 15661663). POLK has 8 isoforms with the molecular mass of 42-99 kDa.

实验方案

Product Specific Protocols
WB protocol for POLK antibody 14455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols