验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14455-1-AP targets POLK in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | POLK fusion protein Ag6034 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA directed) kappa |
| 别名 | DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ |
| 计算分子量 | 99 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC050718 |
| 基因名称 | POLK |
| Gene ID (NCBI) | 51426 |
| RRID | AB_2165839 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBT6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLK, also named as DINB1, belongs to the DNA polymerase type-Y family. POLK is a DNA polymerase specifically involved in DNA repair. POLK Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, POLK inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK is ubiquitously expressed in tissues, but is most abundant in testis, adrenal gland and ovary (PMID: 15661663). POLK has 8 isoforms with the molecular mass of 42-99 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLK antibody 14455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |