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POLQ Polyclonal antibody

POLQ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28590-1-AP

Print datasheet

Synonyms

DKFZp781A0112, DNA polymerase theta variant, Homo sapiens (Human), POLH, POLQ, PRO0327

验证数据展示

  • WB analysis using 28590-1-AP

    WB analysis using 28590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 28590-1-AP

    WB analysis using 28590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 28590-1-AP

    IF Staining of A375 using 28590-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of A431 using 28590-1-AP

    IF Staining of A431 using 28590-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHuH-7 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inA375 cells, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28590-1-AP targets POLQ in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen POLQ fusion protein Ag29643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed), theta
Synonyms DKFZp781A0112, DNA polymerase theta variant, Homo sapiens (Human), POLH, POLQ, PRO0327
Calculated Molecular Weight 290 kDa
Observed Molecular Weight250~290 kDa, 70~110 kDa
GenBank Accession NumberNM_199420
Gene Symbol POLQ
Gene ID (NCBI) 10721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).

实验方案

Product Specific Protocols
WB protocol for POLQ antibody 28590-1-APDownload protocol
IF protocol for POLQ antibody 28590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols