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POLR1A Polyclonal antibody

POLR1A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20595-1-AP

Print datasheet

Synonyms

A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4

验证数据展示

  • WB analysis of HeLa using 20595-1-AP

    WB analysis of HeLa using 20595-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 20595-1-AP

    IP experiment of HeLa using 20595-1-AP

    IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug.

  • WB analysis using 20595-1-AP

    WB analysis using 20595-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

20595-1-AP targets POLR1A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen POLR1A fusion protein Ag14543 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) I polypeptide A, 194kDa
Synonyms A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4
Calculated Molecular Weight 1720 aa, 195 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC017115
Gene Symbol POLR1A
Gene ID (NCBI) 25885
RRIDAB_10949227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

实验方案

Product Specific Protocols
WB protocol for POLR1A antibody 20595-1-APDownload protocol
IP protocol for POLR1A antibody 20595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell

RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination.

Authors - Sijie Liu
View Article
humanIP

Cancer Sci

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Authors - Chaoshen Wu
View Article