验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 1 publications below |
产品信息
20595-1-AP targets POLR1A in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | POLR1A fusion protein Ag14543 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (RNA) I polypeptide A, 194kDa |
| 别名 | A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4 |
| 计算分子量 | 1720 aa, 195 kDa |
| 观测分子量 | 190-195 kDa |
| GenBank蛋白编号 | BC017115 |
| 基因名称 | POLR1A |
| Gene ID (NCBI) | 25885 |
| RRID | AB_10949227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95602 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR1A antibody 20595-1-AP | Download protocol |
| IP protocol for POLR1A antibody 20595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. | ||
Cancer Sci TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC. |
