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POLR1C Polyclonal antibody

POLR1C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15923-1-AP

Print datasheet

Synonyms

AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40

验证数据展示

  • WB analysis using 15923-1-AP

    WB analysis using 15923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 15923-1-AP

    IHC staining of human ovary using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human ovary using 15923-1-AP

    IHC staining of human ovary using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of NIH/3T3 using 15923-1-AP

    IP experiment of NIH/3T3 using 15923-1-AP

    IP result of anti-POLR1C (IP:15923-1-AP, 4ug; Detection:15923-1-AP 1:800) with NIH/3T3 cells lysate 1600 ug.

  • IHC staining of mouse liver using 15923-1-AP

    IHC staining of mouse liver using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15923-1-AP

    IHC staining of mouse liver using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells, HEK-293 cells, Caco-2 cells, HeLa cells, HepG2 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman ovary tissue, mouse liver tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15923-1-AP targets POLR1C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen POLR1C fusion protein Ag8746 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) I polypeptide C, 30kDa
Synonyms AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC008863
Gene Symbol POLR1C
Gene ID (NCBI) 9533
RRIDAB_2167328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR1C is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

实验方案

Product Specific Protocols
WB protocol for POLR1C antibody 15923-1-APDownload protocol
IHC protocol for POLR1C antibody 15923-1-APDownload protocol
IP protocol for POLR1C antibody 15923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
View Article