POLR2H Polyclonal antibody

POLR2H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15086-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

RPABC3, hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, mouse kidney tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15086-1-AP targets POLR2H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 POLR2H fusion protein Ag7166 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide H
别名 RPABC3, hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC000739
基因名称 POLR2H
Gene ID (NCBI) 5437
RRIDAB_2167826
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52434
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

实验方案

Product Specific Protocols
WB protocol for POLR2H antibody 15086-1-APDownload protocol
IHC protocol for POLR2H antibody 15086-1-APDownload protocol
IF protocol for POLR2H antibody 15086-1-APDownload protocol
IP protocol for POLR2H antibody 15086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
humanIHC

Oncol Lett

Identification of the key genes and pathways in prostate cancer.

Authors - Shutong Fan
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
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