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  • Featured Product
  • KD/KO Validated

POLR2I Polyclonal antibody

POLR2I Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17270-1-AP

Print datasheet

Synonyms

hRPB14.5, POLR2I, RNA polymerase II subunit B9, RPB14.5, RPB9

验证数据展示

  • WB analysis using 17270-1-AP

    WB analysis using 17270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17270-1-AP

    IF Staining of HepG2 using 17270-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17270-1-AP (POLR2I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse stomach using 17270-1-AP

    IHC staining of mouse stomach using 17270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLR2I antibody included
  • IHC staining of mouse stomach using 17270-1-AP

    IHC staining of mouse stomach using 17270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLR2I antibody included

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse stomach tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy POLR2I Ready-To-Use IHC Kit. POLR2I primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17270-1-AP targets POLR2I in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen POLR2I fusion protein Ag11027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Synonyms hRPB14.5, POLR2I, RNA polymerase II subunit B9, RPB14.5, RPB9
Calculated Molecular Weight 125 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC017112
Gene Symbol POLR2I
Gene ID (NCBI) 5438
RRIDAB_10836936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

实验方案

Product Specific Protocols
WB protocol for POLR2I antibody 17270-1-APDownload protocol
IHC protocol for POLR2I antibody 17270-1-APDownload protocol
IF protocol for POLR2I antibody 17270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
View Article
humanWB

EMBO Rep

The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy

Authors - Ting Huang
  • KD Validated
View Article