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  • Featured Product
  • KD/KO Validated

POLR2I Polyclonal antibody

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17270-1-AP

Print datasheet

Synonyms

RPB9, RPB14.5, hRPB14.5, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit I

验证数据展示

  • WB analysis using 17270-1-AP

    WB analysis using 17270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 17270-1-AP

    IHC staining of mouse stomach using 17270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLR2I antibody included
  • IHC staining of mouse stomach using 17270-1-AP

    IHC staining of mouse stomach using 17270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with POLR2I antibody included
  • IF Staining of HepG2 using 17270-1-AP

    IF Staining of HepG2 using 17270-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse stomach tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy POLR2I Ready-To-Use IHC Kit. POLR2I primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17270-1-AP targets POLR2I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen POLR2I fusion protein Ag11027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Synonyms RPB9, RPB14.5, hRPB14.5, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit I
Calculated Molecular Weight 125 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC017112
Gene Symbol POLR2I
Gene ID (NCBI) 5438
RRIDAB_10836936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

实验方案

Product Specific Protocols
WB protocol for POLR2I antibody 17270-1-APDownload protocol
IHC protocol for POLR2I antibody 17270-1-APDownload protocol
IF protocol for POLR2I antibody 17270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
View Article
humanWB

EMBO Rep

The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy

Authors - Ting Huang
  • KD Validated
View Article