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POLR3A Polyclonal antibody

POLR3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17530-1-AP

Print datasheet

Synonyms

hRPC155, POLR3A, RNA polymerase III subunit C1, RPC1, RPC155

验证数据展示

  • WB analysis using 17530-1-AP

    WB analysis using 17530-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17530-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 17530-1-AP

    IHC staining of mouse cerebellum using 17530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17530-1-AP targets POLR3A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen POLR3A fusion protein Ag11536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Synonyms hRPC155, POLR3A, RNA polymerase III subunit C1, RPC1, RPC155
Calculated Molecular Weight 1390 aa, 156 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC041089
Gene Symbol POLR3A
Gene ID (NCBI) 11128
RRIDAB_2167983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLR3A antibody 17530-1-APDownload protocol
IHC protocol for POLR3A antibody 17530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Cell Rep

Subcellular localization shapes the fate of RNA polymerase III

Authors - Kai Tian
View Article