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POLR3A Polyclonal antibody

POLR3A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28842-1-AP

Print datasheet

Synonyms

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

验证数据展示

  • WB analysis using 28842-1-AP

    WB analysis using 28842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 28842-1-AP

    IF Staining of A431 using 28842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, Y79 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28842-1-AP targets POLR3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen POLR3A fusion protein Ag29036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Synonyms RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A
Calculated Molecular Weight 1390 aa, 156 kDa
Observed Molecular Weight155 kDa
GenBank Accession NumberBC041089
Gene Symbol POLR3A
Gene ID (NCBI) 11128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).

实验方案

Product Specific Protocols
WB protocol for POLR3A antibody 28842-1-APDownload protocol
IF protocol for POLR3A antibody 28842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols