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POLR3D Polyclonal antibody

POLR3D Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat No : 15339-1-AP

Print datasheet

Synonyms

RPC53, RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T

验证数据展示

  • IP experiment of HeLa using 15339-1-AP

    IP experiment of HeLa using 15339-1-AP

    IP result of anti-POLR3D (IP:15339-1-AP, 4ug; Detection:15339-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • WB analysis using 15339-1-AP

    WB analysis using 15339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15339-1-AP

    IF Staining of HeLa using 15339-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15339-1-AP (POLR3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue, HeLa cells, rat brain tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLR3D Ready-To-Use IHC Kit. POLR3D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15339-1-AP targets POLR3D in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen POLR3D fusion protein Ag7542 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
Synonyms RPC53, RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC004484
Gene Symbol POLR3D
Gene ID (NCBI) 661
RRIDAB_2168013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05423
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

实验方案

Product Specific Protocols
WB protocol for POLR3D antibody 15339-1-APDownload protocol
IF protocol for POLR3D antibody 15339-1-APDownload protocol
IP protocol for POLR3D antibody 15339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FEBS J

Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

Authors - Richa Ahuja
View Article