• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

POLR3F Polyclonal antibody

POLR3F Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16044-1-AP

Print datasheet

Synonyms

RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F

验证数据展示

  • WB analysis of human brain using 16044-1-AP

    WB analysis of human brain using 16044-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16044-1-AP

    IF Staining of HepG2 using 16044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16044-1-AP (POLR3F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, BxPC-3 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16044-1-AP targets POLR3F in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen POLR3F fusion protein Ag8935 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Synonyms RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC012588
Gene Symbol POLR3F
Gene ID (NCBI) 10621
RRIDAB_2165707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1D9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLR3F antibody 16044-1-APDownload protocol
IF protocol for POLR3F antibody 16044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols