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POLR3G Polyclonal antibody

POLR3G Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24701-1-AP

Print datasheet

Synonyms

POLR3G, RNA polymerase III subunit C7, RPC32, RPC7

验证数据展示

  • WB analysis of HeLa using 24701-1-AP

    WB analysis of HeLa using 24701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24701-1-AP

    IP experiment of HeLa using 24701-1-AP

    IP result of anti-POLR3G (IP:24701-1-AP, 3ug; Detection:24701-1-AP 1:500) with HeLa cells lysate 2800ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLR3G Ready-To-Use IHC Kit. POLR3G primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24701-1-AP is POLR3G Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen POLR3G fusion protein Ag19872 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide G (32kD)
Synonyms POLR3G, RNA polymerase III subunit C7, RPC32, RPC7
Calculated Molecular Weight 223 aa, 26 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC141649
Gene Symbol POLR3G
Gene ID (NCBI) 10622
RRIDAB_2879681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.

实验方案

Product Specific Protocols
WB protocol for POLR3G antibody 24701-1-APDownload protocol
IP protocol for POLR3G antibody 24701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder

Authors - Aiko Iwata-Otsubo
View Article