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POLRMT Polyclonal antibody

POLRMT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17748-1-AP

Print datasheet

Synonyms

MTRPOL, MTRNAP, h mtRPOL, EC:2.7.7.6, DNA-directed RNA polymerase, mitochondrial

验证数据展示

  • IF Staining of U2OS using 17748-1-AP

    IF Staining of U2OS using 17748-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POLRMT antibody (17748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 17748-1-AP

    WB analysis using 17748-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17748-1-AP (POLRMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17748-1-AP

    IHC staining of human ovary cancer using 17748-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17748-1-AP (POLRMT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17748-1-AP targets POLRMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen POLRMT fusion protein Ag12012 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) mitochondrial (DNA directed)
Synonyms MTRPOL, MTRNAP, h mtRPOL, EC:2.7.7.6, DNA-directed RNA polymerase, mitochondrial
Calculated Molecular Weight 1230 aa, 139 kDa
Observed Molecular Weight 130-139 kDa
GenBank Accession NumberBC098387
Gene Symbol POLRMT
Gene ID (NCBI) 5442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00411
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial DNA-dependent RNA polymerase (POLRMT) is responsible for the transcription of mitochondrial genome encoding key components of oxidative phosphorylation. Structurally, POLRMT comprises four main domains: the N-terminal extension, a pentatricopeptide repeat (PPR) domain, the N-terminal domain, and the C-terminal domain(PMID: 33602924). POLRMT requires transcription factor B2M (TFB2M) and transcription factor AM (TFAM) for mitochondrial RNA transcription. POLRMT is overexpressed at both the mRNA and protein level in several cancers, including breast, skin, lung, endometrial cancer, and osteosarcomas(PMID: 37371693).

实验方案

Product Specific Protocols
WB protocol for POLRMT antibody 17748-1-APDownload protocol
IHC protocol for POLRMT antibody 17748-1-APDownload protocol
IF protocol for POLRMT antibody 17748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols