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POMC Polyclonal antibody

POMC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22102-1-AP

Print datasheet

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

验证数据展示

  • WB analysis using 22102-1-AP

    WB analysis using 22102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22102-1-AP (POMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse pituitary gland tissue, Transfected HEK-293 cells
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22102-1-AP targets POMC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen POMC fusion protein Ag17311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proopiomelanocortin
Synonyms ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC065832
Gene Symbol POMC
Gene ID (NCBI) 5443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

实验方案

Product Specific Protocols
WB protocol for POMC antibody 22102-1-APDownload protocol
IHC protocol for POMC antibody 22102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols