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验证数据展示

  • WB analysis using 22102-1-AP

    WB analysis using 22102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22102-1-AP (POMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 22102-1-AP

    IF Staining of HeLa using 22102-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (22102-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

POMC Polyclonal antibody

POMC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22102-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CLIP, Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse pituitary gland tissue, Transfected HEK-293 cells
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22102-1-AP targets POMC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen POMC fusion protein Ag17311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proopiomelanocortin
Synonyms CLIP, Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC065832
Gene Symbol POMC
Gene ID (NCBI) 5443
RRIDAB_3085685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

实验方案

Product Specific Protocols
WB protocol for POMC antibody 22102-1-APDownload protocol
IHC protocol for POMC antibody 22102-1-APDownload protocol
IF protocol for POMC antibody 22102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols