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  • Featured Product
  • KD/KO Validated

POMP Polyclonal antibody

POMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15046-1-AP

Print datasheet

Synonyms

PNAS-110, PNAS 110, hUMP1, HSPC036, HSPC014

验证数据展示

  • WB analysis of HeLa using 15046-1-AP

    WB analysis of HeLa using 15046-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15046-1-AP

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • IHC staining of human liver using 15046-1-AP

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 15046-1-AP

    IF Staining of HepG2 using 15046-1-AP

    Immunofluorescent analysis of HepG2 cells, using POMP antibody 15046-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15046-1-AP targets POMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen POMP fusion protein Ag6987 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome maturation protein
Synonyms PNAS-110, PNAS 110, hUMP1, HSPC036, HSPC014
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003390
Gene Symbol POMP
Gene ID (NCBI) 51371
RRIDAB_10858321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POMP antibody 15046-1-APDownload protocol
IHC protocol for POMP antibody 15046-1-APDownload protocol
IF protocol for POMP antibody 15046-1-APDownload protocol
FC protocol for POMP antibody 15046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Dermatol Sci

The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin.

Authors - Barbara A Zieba
  • KD Validated
View Article
mouseWB

JCI Insight

Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.

Authors - Yuki Sasaki
View Article