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验证数据展示

  • WB analysis of HeLa using 15046-1-AP

    WB analysis of HeLa using 15046-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15046-1-AP

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • IHC staining of human liver using 15046-1-AP

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • WB analysis of HepG2 using 15046-1-AP

    WB analysis of HepG2 using 15046-1-AP

    WB result of POMP antibody (15046-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POMP transfected HepG2 cells.

  • IF Staining of HepG2 using 15046-1-AP

    IF Staining of HepG2 using 15046-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using POMP antibody (15046-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

POMP Polyclonal antibody

POMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15046-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

PNAS-110, PNAS 110, hUMP1, HSPC036, HSPC014

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15046-1-AP targets POMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 POMP fusion protein Ag6987 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome maturation protein
别名 PNAS-110, PNAS 110, hUMP1, HSPC036, HSPC014
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC003390
基因名称 POMP
Gene ID (NCBI) 51371
RRIDAB_10858321
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y244
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POMP antibody 15046-1-APDownload protocol
IHC protocol for POMP antibody 15046-1-APDownload protocol
IF protocol for POMP antibody 15046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Dermatol Sci

The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin.

Authors - Barbara A Zieba
  • KD Validated
View Article
mouseWB

JCI Insight

Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.

Authors - Yuki Sasaki
View Article