• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31793-1-AP

    WB analysis using 31793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31793-1-AP (PON3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 31793-1-AP

    IHC staining of rat liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 31793-1-AP

    IHC staining of rat liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31793-1-AP

    IHC staining of mouse liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31793-1-AP

    IHC staining of mouse liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 31793-1-AP

    IHC staining of human liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 31793-1-AP

    IHC staining of human liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PON3 Polyclonal antibody

PON3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31793-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31793-1-AP targets PON3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PON3 fusion protein Ag36602 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paraoxonase 3
别名 Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1
计算分子量NM_000940.2 40kDa，354aa
观测分子量40 kDa
GenBank蛋白编号NM_000940.2
基因名称 PON3
Gene ID (NCBI) 5446
RRIDAB_3670115
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ15166
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.

实验方案

Product Specific Protocols
WB protocol for PON3 antibody 31793-1-APDownload protocol
IHC protocol for PON3 antibody 31793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols