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POP1 Polyclonal antibody
POP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | POP1 fusion protein Ag2660 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae) |
Synonyms | |
Calculated Molecular Weight | 1024 aa, 115 kDa |
Observed Molecular Weight | 115 kDa |
GenBank Accession Number | BC011529 |
Gene Symbol | POP1 |
Gene ID (NCBI) | 10940 |
RRID | AB_2166477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99575 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for POP1 antibody 12029-1-AP | Download protocol |
IF protocol for POP1 antibody 12029-1-AP | Download protocol |
IP protocol for POP1 antibody 12029-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Research (Wash D C) POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA | ||
Cell Rep m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation
|