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  • KD/KO Validated

POP1 Polyclonal antibody

POP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12029-1-AP

Print datasheet

Synonyms



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen POP1 fusion protein Ag2660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
Synonyms
Calculated Molecular Weight 1024 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC011529
Gene Symbol POP1
Gene ID (NCBI) 10940
RRIDAB_2166477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.

实验方案

Product Specific Protocols
WB protocol for POP1 antibody 12029-1-APDownload protocol
IF protocol for POP1 antibody 12029-1-APDownload protocol
IP protocol for POP1 antibody 12029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III

Authors - Sergio Leone
humanWB

Mol Cell

Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.

Authors - Ok Hyun Park
humanWB

Nat Microbiol

The SARS-CoV-2 RNA-protein interactome in infected human cells.

Authors - Nora Schmidt
humanWB,RIP,IHC

Research (Wash D C)

POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA

Authors - Chao Zhang
humanWB

Cell Rep

UPF1 promotes rapid degradation of m6A-containing RNAs.

Authors - Sung Ho Boo
humanIP,WB

Cell Rep

m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation

Authors - Sung Ho Boo
  • KD Validated