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POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15273-1-AP

Print datasheet

Synonyms

RPP29, POP 4, hPOP4

验证数据展示

  • WB analysis of HepG2 using 15273-1-AP

    WB analysis of HepG2 using 15273-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15273-1-AP

    IF Staining of HepG2 using 15273-1-AP

    Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen POP4 fusion protein Ag7387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
Synonyms RPP29, POP 4, hPOP4
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC004438
Gene Symbol POP4
Gene ID (NCBI) 10775
RRIDAB_2284033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95707
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POP4 antibody 15273-1-APDownload protocol
IF protocol for POP4 antibody 15273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols