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POP7 Polyclonal antibody

POP7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14964-1-AP

Print datasheet

Synonyms

RPP20, RPP2, RNaseP protein p20, hPOP7

验证数据展示

  • WB analysis of HeLa using 14964-1-AP

    WB analysis of HeLa using 14964-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14964-1-AP (POP7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14964-1-AP

    IP experiment of HeLa using 14964-1-AP

    IP result of anti-POP7 (IP:14964-1-AP, 4ug; Detection:14964-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IF Staining of HeLa using 14964-1-AP

    IF Staining of HeLa using 14964-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14964-1-AP (POP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14964-1-AP targets POP7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen POP7 fusion protein Ag6827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae)
Synonyms RPP20, RPP2, RNaseP protein p20, hPOP7
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC001430
Gene Symbol POP7
Gene ID (NCBI) 10248
RRIDAB_2166488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POP7 antibody 14964-1-APDownload protocol
IF protocol for POP7 antibody 14964-1-APDownload protocol
IP protocol for POP7 antibody 14964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article