验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue, human skeletal muscle tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
产品信息
11800-1-AP targets POPDC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | POPDC3 fusion protein Ag2375 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | popeye domain containing 3 |
| 别名 | POP3, POPDC3, popeye domain containing 3, Popeye protein 3, RP11 99L11.2 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC022323 |
| 基因名称 | POPDC3 |
| Gene ID (NCBI) | 64208 |
| RRID | AB_2166494 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBV1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| IHC protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| IP protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Carcinogenesis Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.
| ||
Front Oncol Study on differentially expressed genes between stage M and stage MS neuroblastoma | ||
World J Gastroenterol Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer. |
