POT1 Polyclonal antibody

POT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10581-1-AP

Print datasheet

Synonyms

hPot1, POT1



经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10581-1-AP targets POT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen POT1 fusion protein Ag0875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POT1 protection of telomeres 1 homolog (S. pombe)
Synonyms hPot1, POT1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC002923
Gene Symbol POT1
Gene ID (NCBI) 25913
RRIDAB_2284057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)

实验方案

Product Specific Protocols
WB protocol for POT1 antibody 10581-1-APDownload protocol
IHC protocol for POT1 antibody 10581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients.

Authors - Jianwei Chen
humanIP

Cell Cycle

CGGBP1 phosphorylation constitutes a telomere-protection signal.

Authors - Umashankar Singh
humanWB

Sci Rep

Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer.

Authors - Hui Yang
humanWB

Front Cell Dev Biol

Telomere Attrition in Induced Pluripotent Stem Cell-Derived Neurons From ALS/FTD-Related C9ORF72 Repeat Expansion Carriers.

Authors - Hayley Robinson
humanWB

J Cancer

Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.

Authors - Hui Yang
mouseIHC

Exp Gerontol

The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse.

Authors - Esra Gozde Kosebent