POT1 Polyclonal antibody
POT1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, PC-3 cells |
Positive IHC detected in | human prostate cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10581-1-AP targets POT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | POT1 fusion protein Ag0875 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | POT1 protection of telomeres 1 homolog (S. pombe) |
Synonyms | hPot1, POT1 |
Calculated Molecular Weight | 71 kDa |
Observed Molecular Weight | 71 kDa |
GenBank Accession Number | BC002923 |
Gene Symbol | POT1 |
Gene ID (NCBI) | 25913 |
RRID | AB_2284057 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NUX5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for POT1 antibody 10581-1-AP | Download protocol |
IHC protocol for POT1 antibody 10581-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Aging (Albany NY) SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients. | ||
Sci Rep Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer. | ||
Front Cell Dev Biol Telomere Attrition in Induced Pluripotent Stem Cell-Derived Neurons From ALS/FTD-Related C9ORF72 Repeat Expansion Carriers. | ||
J Cancer Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells. | ||
Exp Gerontol The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse. |