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验证数据展示

  • IHC staining of human ovary cancer using 24593-1-AP

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 24593-1-AP

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 24593-1-AP

    WB analysis of human testis using 24593-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24593-1-AP (POTEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 24593-1-AP

    IHC staining of human ovary using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24593-1-AP (POTEA Antibody) at dilution of 1:50 (under 40x lens).

POTEA Polyclonal antibody

POTEA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24593-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

POTE-8, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24593-1-AP targets POTEA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 POTEA fusion protein Ag20228 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POTE ankyrin domain family, member A
别名 POTE-8, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1
计算分子量 498 aa, 56 kDa
观测分子量 53 kDa
GenBank蛋白编号BC153076
基因名称 POTEA
Gene ID (NCBI) 340441
RRIDAB_2879627
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6S8J7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA also named as ANKRD26 like family A member 1 or POTE8 is a 498 amino acid protein, which contains five ANK repeats and belongs to the POTE family. POTEA has an important signaling function in the reproductive system.

实验方案

Product Specific Protocols
WB protocol for POTEA antibody 24593-1-APDownload protocol
IHC protocol for POTEA antibody 24593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols