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POTEA Polyclonal antibody

POTEA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24593-1-AP

Print datasheet

Synonyms

POTE-8, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

验证数据展示

  • IHC staining of human ovary cancer using 24593-1-AP

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 24593-1-AP

    IHC staining of human ovary cancer using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 24593-1-AP (POTEA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 24593-1-AP

    WB analysis of human testis using 24593-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24593-1-AP (POTEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 24593-1-AP

    IHC staining of human ovary using 24593-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24593-1-AP (POTEA Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman testis tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24593-1-AP targets POTEA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen POTEA fusion protein Ag20228 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POTE ankyrin domain family, member A
Synonyms POTE-8, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1
Calculated Molecular Weight 498 aa, 56 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC153076
Gene Symbol POTEA
Gene ID (NCBI) 340441
RRIDAB_2879627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6S8J7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA also named as ANKRD26 like family A member 1 or POTE8 is a 498 amino acid protein, which contains five ANK repeats and belongs to the POTE family. POTEA has an important signaling function in the reproductive system.

实验方案

Product Specific Protocols
WB protocol for POTEA antibody 24593-1-APDownload protocol
IHC protocol for POTEA antibody 24593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols