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POU2AF1 Polyclonal antibody

POU2AF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13605-1-AP

Print datasheet

Synonyms

BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1

验证数据展示

  • WB analysis of mouse lung using 13605-1-AP

    WB analysis of mouse lung using 13605-1-AP

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

  • WB analysis using 13605-1-AP

    WB analysis using 13605-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, mouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13605-1-AP targets POU2AF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen POU2AF1 fusion protein Ag4482 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POU class 2 associating factor 1
Synonyms BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1
Calculated Molecular Weight 256 aa, 27 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC032549
Gene Symbol POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2252633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

实验方案

Product Specific Protocols
WB protocol for POU2AF1 antibody 13605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols