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验证数据展示

  • WB analysis of mouse lung using 13605-1-AP

    WB analysis of mouse lung using 13605-1-AP

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

  • WB analysis using 13605-1-AP

    WB analysis using 13605-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

POU2AF1 Polyclonal antibody

POU2AF1 Polyclonal Antibody for WB, ELISA
Cat No. 13605-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, mouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13605-1-AP targets POU2AF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 POU2AF1 fusion protein Ag4482 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POU class 2 associating factor 1
别名 BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1
计算分子量 256 aa, 27 kDa
观测分子量 35 kDa
GenBank蛋白编号BC032549
基因名称 POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2252633
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16633
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

实验方案

Product Specific Protocols
WB protocol for POU2AF1 antibody 13605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols