BRN2-Specific Polyclonal antibody

BRN2-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 18998-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA and More (1)

BRN2, POU3F2, Brn-2, Brn 2, Brain-specific homeobox/POU domain protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy POU3F2/BRN2 Ready-To-Use IHC Kit. POU3F2/BRN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18998-1-AP targets BRN2-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POU class 3 homeobox 2
别名 BRN2, POU3F2, Brn-2, Brn 2, Brain-specific homeobox/POU domain protein 2
计算分子量 47 kDa
观测分子量 47-54 kDa
GenBank蛋白编号NM_005604
基因名称 BRN2
Gene ID (NCBI) 5454
RRIDAB_10597389
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20265
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

实验方案

Product Specific Protocols
WB protocol for BRN2-Specific antibody 18998-1-APDownload protocol
IHC protocol for BRN2-Specific antibody 18998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genes Dev

Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas.

Authors - Justin P Norton
ratWB

CNS Neurosci Ther

Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis

Authors - Nesrine Saeid El-Mezayen
IF

Elife

Human-specific ARHGAP11B induces hallmarks of neocortical expansion in developing ferret neocortex.

Authors - Nereo Kalebic
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