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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

    WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

    IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

    IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POU4F3 antibody included
  • FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

    FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 82980-1-RR (same clone as 82980-1-PBS)

    IF Staining of rat brain using 82980-1-RR (same clone as 82980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82980-1-RR

    Affinity and Kinetic Characterization of 82980-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

POU4F3 Recombinant antibody, PBS Only

POU4F3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 82980-1-PBS

产品说明书

CloneNo. 230345F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

BRN3C, Brn 3C, Brain-specific homeobox/POU domain protein 3C, Brain-3C, Brain 3C

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy POU4F3 Ready-To-Use IHC Kit. POU4F3 primary antibody included.

产品信息

82980-1-PBS targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 POU4F3 fusion protein Ag16004 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 POU class 4 homeobox 3
别名 BRN3C, Brn 3C, Brain-specific homeobox/POU domain protein 3C, Brain-3C, Brain 3C
计算分子量 338 aa, 37 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC104923
基因名称 POU4F3
Gene ID (NCBI) 5459
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15319
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C