• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PPA1 Polyclonal antibody

PPA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14985-1-AP

Print datasheet

Synonyms

PPase, PP1, PP, IOPPP, Inorganic pyrophosphatase

验证数据展示

  • IF Staining of HeLa using 14985-1-AP

    IF Staining of HeLa using 14985-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human heart using 14985-1-AP

    WB analysis of human heart using 14985-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 14985-1-AP

    IP experiment of mouse liver using 14985-1-AP

    IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

  • IHC staining of human breast cancer using 14985-1-AP

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14985-1-AP

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue, human liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14985-1-AP targets PPA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPA1 fusion protein Ag6969 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrophosphatase (inorganic) 1
Synonyms PPase, PP1, PP, IOPPP, Inorganic pyrophosphatase
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001022
Gene Symbol PPA1
Gene ID (NCBI) 5464
RRIDAB_2167890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15181
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

实验方案

Product Specific Protocols
WB protocol for PPA1 antibody 14985-1-APDownload protocol
IHC protocol for PPA1 antibody 14985-1-APDownload protocol
IF protocol for PPA1 antibody 14985-1-APDownload protocol
IP protocol for PPA1 antibody 14985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Metabolites

PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening

Authors - Melanie T Achleitner
View Article
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article