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验证数据展示

  • IF Staining of HeLa using 14985-1-AP

    IF Staining of HeLa using 14985-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human heart using 14985-1-AP

    WB analysis of human heart using 14985-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 14985-1-AP

    IP experiment of mouse liver using 14985-1-AP

    IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

  • IHC staining of human breast cancer using 14985-1-AP

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14985-1-AP

    IHC staining of human breast cancer using 14985-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPA1 Polyclonal antibody

PPA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14985-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PPase, PP1, PP, IOPPP, Inorganic pyrophosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, human liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14985-1-AP targets PPA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPA1 fusion protein Ag6969 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrophosphatase (inorganic) 1
别名 PPase, PP1, PP, IOPPP, Inorganic pyrophosphatase
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001022
基因名称 PPA1
Gene ID (NCBI) 5464
RRIDAB_2167890
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

实验方案

Product Specific Protocols
WB protocol for PPA1 antibody 14985-1-APDownload protocol
IHC protocol for PPA1 antibody 14985-1-APDownload protocol
IF protocol for PPA1 antibody 14985-1-APDownload protocol
IP protocol for PPA1 antibody 14985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Metabolites

PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening

Authors - Melanie T Achleitner
View Article
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article