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验证数据展示

  • WB analysis using 68178-1-Ig

    WB analysis using 68178-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68178-1-Ig (PPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68178-1-Ig

    IF Staining of HeLa using 68178-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PPA1 antibody (68178-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PPA1 Monoclonal antibody

PPA1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68178-1-Ig

产品说明书

CloneNo. 1D11F4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat

应用

WB, IF/ICC, ELISA

别名

Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68178-1-Ig targets PPA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Rat
免疫原 PPA1 fusion protein Ag7049 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrophosphatase (inorganic) 1
别名 Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061
计算分子量 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC001022
基因名称 PPA1
Gene ID (NCBI) 5464
RRIDAB_2935269
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

实验方案

Product Specific Protocols
WB protocol for PPA1 antibody 68178-1-IgDownload protocol
IF protocol for PPA1 antibody 68178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols