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PPA2 Polyclonal antibody

PPA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16662-1-AP

Print datasheet

Synonyms

HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306

验证数据展示

  • WB analysis of mouse heart using 16662-1-AP

    WB analysis of mouse heart using 16662-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • WB analysis using 16662-1-AP

    WB analysis using 16662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPA2 Ready-To-Use IHC Kit. PPA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16662-1-AP targets PPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPA2 fusion protein Ag10138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrophosphatase (inorganic) 2
Synonyms HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306
Calculated Molecular Weight305aa,35 kDa; 334aa,38 kDa
Observed Molecular Weight 32-40 kDa
GenBank Accession NumberBC057219
Gene Symbol PPA2
Gene ID (NCBI) 27068
RRIDAB_1851549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2U2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

实验方案

Product Specific Protocols
WB protocol for PPA2 antibody 16662-1-APDownload protocol
IHC protocol for PPA2 antibody 16662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Multi-omics pan-cancer analysis reveals the prognostic values and immunological functions of PPA2, with a spotlight on breast cancer

Authors - Jia-Ning Zhang
View Article