验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, rat heart tissue, rat liver tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPAN Ready-To-Use IHC Kit. PPAN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11006-1-AP targets PPAN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, xenopus |
| 免疫原 | PPAN fusion protein Ag1419 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peter pan homolog (Drosophila) |
| 别名 | BXDC3, Peter Pan homolog, peter pan homolog (Drosophila), PPAN, SSF, Ssf 1, SSF1, SSF2, Suppressor of SWI4 1 homolog |
| 计算分子量 | 53 kDa |
| 观测分子量 | 55-58 kDa |
| GenBank蛋白编号 | BC009833 |
| 基因名称 | PPAN |
| Gene ID (NCBI) | 56342 |
| RRID | AB_2168040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQ55 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPAN antibody 11006-1-AP | Download protocol |
| IHC protocol for PPAN antibody 11006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. | ||
Development Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis. | ||
Cancers (Basel) Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.
| ||
J Biol Chem The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.
| ||
Biol Cell Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development. | ||
Cells Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.
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