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- KD/KO Validated
PPAN Polyclonal antibody
PPAN Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, rat heart tissue, rat liver tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPAN Ready-To-Use IHC Kit. PPAN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11006-1-AP targets PPAN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus |
| Immunogen | PPAN fusion protein Ag1419 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | peter pan homolog (Drosophila) |
| Synonyms | BXDC3, Peter Pan homolog, peter pan homolog (Drosophila), PPAN, SSF, Ssf 1, SSF1, SSF2, Suppressor of SWI4 1 homolog |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 55-58 kDa |
| GenBank Accession Number | BC009833 |
| Gene Symbol | PPAN |
| Gene ID (NCBI) | 56342 |
| RRID | AB_2168040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQ55 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPAN antibody 11006-1-AP | Download protocol |
| IHC protocol for PPAN antibody 11006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. | ||
Development Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis. | ||
Cancers (Basel) Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.
| ||
J Biol Chem The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.
| ||
Biol Cell Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development. | ||
Cells Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.
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